杜亚瑞,邵 娜

目的：基于网络药理学与分子对接预测柴胡疏肝散干预缺血性脑卒中（IS）的活性成分、核心靶点及关键通路。方法：经TCMSP、BATMAN-TCM及TCMID获取组方成分，以OB≥30%、DL≥0.18筛选活性成分并预测靶点；自GeneCards等库收集IS靶点并取交集；以Cytoscape、STRING构建网络筛选核心靶点，行GO与KEGG富集分析，并以AutoDock Vina行分子对接。结果：获活性成分164个、交集靶点248个；槲皮素、山柰酚、β-胡萝卜素、柚皮素及β-谷甾醇为关键成分，TP53、IL1B、TNF、AKT1及IL6为核心靶点；显著富集于PI3K-Akt、VEGF、AGE-RAGE、TNF及凋亡通路；结合能普遍较低（最低-11.42 kcal/mol）。结论：柴胡疏肝散可能经多成分-多靶点-多通路协同调控炎症、凋亡、氧化应激及血管新生而干预IS。

柴胡疏肝散；缺血性脑卒中；网络药理学；分子对接

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