赵永胜,周 锐,张 娟,马小茶,师卫华

目的：探讨跨膜蛋白16A（TMEM16A/ANO1）在乳腺癌中的表达及其对癌细胞增殖、周期、凋亡、侵袭及体内成瘤的影响。方法：收集20例乳腺癌及配对癌旁组织检测TMEM16A表达；MCF-7细胞中敲低TMEM16A后检测增殖、周期、凋亡与侵袭；裸鼠移植瘤模型观察体内成瘤并检测MAPK通路蛋白及Cyclin D1。结果：乳腺癌组织中TMEM16A mRNA和蛋白均高表达（P<0.01）。敲低TMEM16A可抑制MCF-7细胞增殖、诱导G0/G1期阻滞、削弱侵袭（P<0.01），对凋亡影响不明显；移植瘤生长明显减缓（P<0.05），伴p-p38、p-ERK1/2及Cyclin D1下调，总蛋白无明显变化。结论：TMEM16A在乳腺癌中高表达，敲低后可能经p38/ERK1/2-Cyclin D1轴抑制肿瘤细胞增殖、侵袭及体内成瘤，是潜在治疗靶点。

乳腺癌；TMEM16A；细胞周期；细胞侵袭；MAPK信号通路；Cyclin D1

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