陈男雄

目的：探讨Regesi再生硅对兔耳增生性瘢痕的干预效果及其调控成纤维细胞活化的机制。方法：建立兔耳增生性瘢痕模型，分为空白对照组、商品硅凝胶组和Regesi再生硅组，动态观察瘢痕外观，并检测瘢痕面积、增生指数、横截面积及抬高指数。体外以TGF-β1诱导成纤维细胞活化，观察Regesi再生硅对细胞增殖、迁移、α-SMA、Collagen I、胶原沉积及相关信号蛋白的影响。结果：Regesi再生硅可减轻兔耳瘢痕红肿、隆起和组织增厚，降低瘢痕面积及增生相关指标；体外可抑制TGF-β1诱导的成纤维细胞增殖、迁移、肌成纤维分化和胶原沉积，并下调p-Smad2/3、p-Smad1/5/8、p-ERK1/2、p-p38及p-AKT表达。结论：Regesi再生硅可能通过抑制TGF-β1介导的Smad及Non-Smad通路，减轻成纤维细胞活化和细胞外基质沉积，具有外用抗瘢痕应用潜力。

Regesi再生硅；增生性瘢痕；兔耳模型；成纤维细胞；TGF-β1；Smad信号通路

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