张艳艳,彭银艳,马嘉骏,席亚慧,邵雪丽

目的：探讨脐带间充质干细胞来源外泌体（hUCMSC-Exos）对糖尿病性角膜病变（DK）的保护作用及对焦亡核心因子Caspase-1的调控机制。方法：选取SPF级SD大鼠，采用链脲佐菌素（STZ）腹腔注射构建糖尿病大鼠模型，随机分为正常对照组、糖尿病模型组、hUCMSC-Exos干预组。干预组给予hUCMSC-Exos点眼干预。分别采用角膜共聚焦显微镜观察角膜结构，虎红染色评估角膜上皮细胞损伤与通透性改变，荧光素钠染色评估角膜上皮连续性与缺损范围；采用qRT-PCR检测角膜组织中焦亡核心基因Caspase-1的mRNA表达水平。结果：与对照组相比，糖尿病组大鼠角膜虎红染色、荧光素钠染色评分显著升高，角膜上皮完整性破坏明显，Caspase-1表达显著上调；与糖尿病组相比，hUCMSC-Exos干预组大鼠角膜虎红染色强度明显降低，荧光素钠染色缺损面积显著缩小，Caspase-1表达显著下调。结论：hUCMSC-Exos可有效改善糖尿病性角膜病变，其机制与抑制焦亡核心分子Caspase-1的过度激活密切相关。

糖尿病性角膜病变；脐带间充质干细胞；外泌体；细胞焦亡；Caspase-1；角膜上皮损伤

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