陈诗少,兰 想,袁昱微,何国珍,黎明星

目的：探讨温胆汤含药血清抗皮质酮损伤HT22细胞的分子机制。方法：选50只SD大鼠，随机分为对照组和温胆汤低、中、高剂量组，制备相应血清。以皮质酮损伤HT22细胞为模型，与血清共培养24h后，CCK-8、Western blot和Q-PCR分别检测细胞活力、BDNF蛋白和mRNA的表达水平。结果：模型组（皮质酮损伤组）细胞活力、BDNF的蛋白和mRNA表达水平较正常组明显下降；温胆汤含药血清中剂量组细胞活力、BDNF mRNA表达水平、各剂量组BDNF蛋白表达水平较模型组显著上升。结论：温胆汤含药血清具有抗皮质酮损伤神经元的作用，其机制可能是通过调控BDNF的表达来实现。

温胆汤含药血清；HT22细胞；BDNF

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