周 曦,成 航,李 鹏

噪声性听力损失由不可逆的耳蜗毛细胞损伤引起，由于对内源性保护机制了解有限，目前缺乏有效的治疗方法。本研究旨在探讨Drp1在噪声性听力损失发病中的作用及其机制。通过建立小鼠噪声暴露模型，采用qRT-PCR、听觉脑干反应检测、ELISA及蛋白质印迹法等技术进行分析。结果显示，噪声暴露可显著上调耳蜗组织中Drp1的mRNA表达；Drp1抑制剂Mdivi-1能有效降低噪声暴露后各时间点的ABR阈值，减轻听功能损伤。进一步机制研究表明，Mdivi-1干预可显著改善氧化应激，降低MDA水平、提高SOD活性并调节TAC水平，同时逆转噪声引起的Bax/Bcl-2比值升高及Caspase-3活化。结果表明，Drp1通过促进氧化应激及激活线粒体依赖性凋亡通路参与噪声性耳聋的发病过程，抑制Drp1可能成为防治该病的潜在策略。

噪声性听力损失；线粒体动力相关蛋白1；细胞凋亡

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