冯 伟,路吉坤

研究表明，源自牙周膜（PDL）细胞的高迁移率族蛋白 B1（HMGB1）可能是正畸牙齿移动和牙周组织重塑过程中的潜在调节因子。本研究旨在采用Waldo 法对小鼠磨牙进行持续加力，探究小鼠正畸牙齿移动过程中牙周组织中HMGB1的表达情况。实验选用6只7周龄的C57BL6 小鼠，将弹性带置于右上颌第一和第二磨牙之间的牙缝中，施加机械力3天后，用含4%多聚甲醛的0.1M磷酸缓冲液（pH7.4）经心脏灌注固定小鼠，然后取出上颌骨进行组织化学分析。组织学检查显示，张力侧出现局部牙周膜撕裂，压迫侧形成广泛的无细胞透明带。HMGB1的免疫定位在牙周膜张力侧、根尖区和牙髓中显著呈现，而在牙周膜压迫侧，由于透明带的存在，HMGB1的标记几乎检测不到。综上所述，我们得出结论，采用Waldo法进行的正畸牙齿移动会导致与螺旋弹簧法不同的组织学变化和HMGB1 表达模式，包括牙周膜撕裂和广泛的透明带形成，这可能会严重破坏牙周组织，进而阻碍牙齿移动。

HMGB1；正畸牙齿移动；牙周组织；Waldo法

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