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目的：利用公共生物信息学数据库筛选出与宫颈鳞状细胞癌（cervical squamous cell carcinoma，CSCC）相关的关键基因，分析这些基因在肿瘤发生发展过程中所起的作用。方法：从Gene Expression Omnibus（GEO）数据库下载CSCC相关基因表达数据集GSE29570和GSE63514，利用GEO2R在线分析工具筛选差异表达基因（DEGs），以P.adj＜0.05且|LogFC|＞1为筛选条件，并通过韦恩图获得两个数据集的交集基因。结果：GEO数据库分析得到340个交集差异表达基因，上调基因231个，下调基因103个。结论：NEK2可以促进糖酵解途径来提高肿瘤细胞的增殖能力，进而影响患者的预后。NEK2可以成为CSCC的预后生物标志物和分子治疗靶点，给宫颈鳞癌的机制研究以及靶向治疗提供新的研究思路。

宫颈鳞癌；NEK2；GEO数据库；糖酵解；生物信息学分析

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