刘 涛,李长虹

背景：长链非编码RNA（Long non-coding RNA，LncRNA）在肝细胞癌（hepatocellular carcinoma，HCC）的发生发展中发挥重要作用。本研究旨在探讨HOTAIR在HCC中的表达模式及其调控肿瘤转移的具体机制。方法：采用Northern blot检测临床样本中HOTAIR的水平；通过Western blot分析HOTAIR、DNMTs与PTEN之间的调控关系；利用划痕试验和Transwell实验评估细胞的迁移与侵袭能力。结果：HOTAIR在肝癌组织中显著高表达。HOTAIR能够上调DNMT1和DNMT3A的表达，进而抑制抑癌基因PTEN的水平。过表达HOTAIR显著增强了肝细胞的迁移和侵袭能力，而这一效应可被DNMT1的敲低所逆转。回复实验证实，敲低DNMT1可显著逆转HOTAIR引起的PTEN下调及细胞迁移能力的增强。结论：HOTAIR通过DNMT1/PTEN轴促进肝癌的进展，可能作为肝癌治疗的潜在靶点。

LncRNA HOTAIR；DNMT1；PTEN；肝细胞癌

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