李雯婷,程佩瑶,杨筱舟

目的：评估三种附子炮制品（黑顺片、白附片、盐附子）对阿尔茨海默病（AD）模型秀丽隐杆线虫的神经保护作用及其潜在作用机制。方法：采用体外抗氧化实验检测其对ABTS⁺、羟基自由基的清除能力，结合线虫24 h急性毒性、寿命、繁殖能力、瘫痪实验，及硫磺素S染色、运动速率、急性氧化应激和SOD活性检测，系统评价附子炮制品的抗氧化活性与神经保护效应。结果：三种附子炮制品均表现出显著的浓度依赖性抗氧化活性，能延长AD模型线虫的生存期、改善其运动功能，且可部分抑制线虫体内Aβ蛋白积累；急性毒性实验未发现明显不良反应，在适宜浓度下还能显著延缓线虫衰老进程、提升其繁殖能力。结论：附子炮制品可能通过抗氧化应激机制缓解AD模型线虫的相关病理症状，在AD治疗中展现出一定的潜在应用价值，但其具体作用靶点与临床疗效仍需进一步深入验证。

阿尔茨海默病；附子；秀丽隐杆线虫；氧化应激；神经保护

[1] Scheltens P,De Strooper B,Kivipelto M,et al.Alzheimer's disease[J].Lancet,2021,397(10284):1577-1590. [2] Liu JY,Guo HY,Quan ZS,et al.Research progress of natural products and their derivatives against Alzheimer's disease[J].J Enzyme Inhib Med Chem,2023,38(1):2171026. [3] Jucker M,Walker LC.Alzheimer's disease:From immunotherapy to immunoprevention[J].Cell,2023,186(20):4260-4270. [4] Zhang W,Lu C,Cai S,et al.Aconiti Lateralis Radix Praeparata as potential anticancer herb:Bioactive compounds and molecular mechanisms[J].Front Pharmacol,2022,13:870282. [5] Wu Z,Qian J,Feng C,et al.A review of Aconiti Lateralis Radix Praeparata(Fuzi)for kidney disease:Phytochemistry,toxicology,herbal processing,and pharmacology[J].Front Pharmacol,2024,15:1427333. [6] Chan YT,Wang N,Feng Y.The toxicology and detoxification of Aconitum:Traditional and modern views[J].Chin Med,2021,16(1):61. [7] Liu X,Xie X,Luo M,et al.The synergistic compatibility mechanisms of fuzi against chronic heart failure in animals:A systematic review and meta-analysis[J].Front Pharmacol,2022,13:954253. [8] Tai CJ,El-Shazly M,Tsai YH,et al.Uncovering modern clinical applications of fuzi and fuzi-based formulas:A nationwide descriptive study with market basket analysis[J].Front Pharmacol,2021,12:641530. [9] 邹天赐,姚礼平,席娜.针刺加麻黄附子细辛汤加减联合西药治疗三叉神经痛的研究[J].中医研究,2023,36(10):62-65. Zou Tianci,Yao Liping,Xi Na.Study on acupuncture combined with modified Mahuang Fuzi Xixin decoction and western medicine in the treatment of trigeminal neuralgia[J].Research of Traditional Chinese Medicine,2023,36(10):62-65. [10] Xiang T,Yang X,Zhang X,et al.Processing reduces diester diterpenoid alkaloids content of fuzi products,resulting in reduced toxicity and modified bioactivities[J].J Nat Med,2025,79(3):695-705. [11] Huang ZQ,Chen P,Su WW,et al.Antioxidant activity and hepatoprotective potential of quercetin 7-rhamnoside in vitro and in vivo[J].Molecules,2018,23(5):1188. [12] Halliwell B,Gutteridge JM.Free radicals in biology and medicine[M].Oxford:Oxford University Press,2015. [13] Acosta C,Barat JM,Martínez-Máñez R,et al.Toxicological assessment of mesoporous silica particles in the nematode Caenorhabditis elegans[J].Environ Res,2018,166:61-70. [14] Zheng F,Chen C,Aschner M.Neurotoxicity evaluation of nanomaterials using C.elegans:Survival,locomotion behaviors,and oxidative stress[J].Curr Protoc,2022,2(7):e496. [15] Duxbury EML,Carlsson H,Sales K,et al.Multigenerational downregulation of insulin/IGF-1 signaling in adulthood improves lineage survival,reproduction,and fitness in Caenorhabditis elegans[J].Evolution,2022,76(12):2829-2845. [16] Tan X,Xu R,Li AP,et al.Antioxidant and anti-Alzheimer's disease activities of 1,8-cineole and its cyclodextrin inclusion complex[J]. Biomed Pharmacother,2024,175:116784. [17] Kanteti R,Dhanasingh I,El-Hashani E,et al.C.elegans and mutants with chronic nicotine exposure as a novel model of cancer phenotype[J].Cancer Biol Ther,2016,17(1):91-103. [18] Wen YP,Fu HJ,Chen Q,et al.Exploring the therapeutic potential of Nelumbo nucifera leaf extract against amyloid-beta-induced toxicity in the Caenorhabditis elegans model of Alzheimer's disease[J].Front Pharmacol,2024,15:1408031.

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