沈祥礼,张亚豪,菲尔凯提·玉山江,张保俭,姜述斌

目的：探讨雪莲注射液调控巨噬细胞极化改善心肌缺血再灌注损伤的作用。方法：50只健康雄性C57BL/6小鼠随机分为假手术组、模型组、雪莲注射液低、中、高剂量组。假手术组开胸、穿线但不结扎冠状动脉，模型组和雪莲注射液低、中、高剂量组通过结扎冠状动脉构建MIRI模型。雪莲注射液低、中、高剂量组注射相应浓度的雪莲注射液，假手术组和模型组注射等体积0.9%氯化钠注射液。连续干预7d后，流式细胞术（FCM）测定M1、M2型巨噬细胞占比，酶联免疫吸附（ELISA）法检测血清TNF-α、IL-6、IL-10、TGF-β水平。结果：与假手术组相比，模型组小鼠的M1型巨噬细胞比例及M1/M2比值显著升高（P<0.01），而M2型巨噬细胞比例显著降低（P<0.01）；同时，血清促炎因子IL-6、TNF-α水平显著升高（P<0.01），抗炎因子IL-10、TGF-β水平显著降低（P<0.01）。与模型组相比，雪莲注射液各剂量组可显著逆转上述变化，表现为M1型巨噬细胞比例及M1/M2比值降低（P<0.01），M2型巨噬细胞比例升高（P<0.01）；同时，IL-6与TNF-α水平显著下降（P<0.01），而IL-10与TGF-β水平显著升高（P<0.01）。结论：心肌缺血再灌注损伤小鼠可促进巨噬细胞向M1型极化，并增加炎症反应，雪莲注射液可使巨噬细胞向M2型极化，进而缓解炎症反应。

雪莲注射液；心肌缺血再灌注损伤；巨噬细胞极化

[1] Spray L,Richardson G,Haendeler J,et al.Cardiovascular inflammaging:Mechanisms,consequences,and therapeutic perspectives.Cell Rep Med.2025;6(9):102264. [2] Ziegler KA,Engelhardt S,Carnevale D,et al.Neural Mechanisms in Cardiovascular Health and Disease.Circ Res.2025;136(11):1233-1261. [3] Chapman AR,Taggart C,Boeddinghaus J,et al.Type 2 myocardial infarction:challenges in diagnosis and treatment.Eur Heart J.2025; 46(6):504-517. [4] Xiang Q,Yi X,Zhu XH,et al.Regulated cell death in myocardial ischemia-reperfusion injury.Trends Endocrinol Metab.2024;35(3):219-234. [5] Algoet M,Janssens S,Himmelreich U,et al.Myocardial ischemia-reperfusion injury and the influence of inflammation.Trends Cardiovasc Med.2023;33(6):357-366. [6] Du H,You X,Zhang J,et al.CAR Macrophages Engineered In Vivo for Attenuating Myocardial Ischemia-Reperfusion Injury.Circ Res. 2025;137(6):846-859. [7] Chen DX,Feng YY,Wang HY,et al.Metrnl ameliorates myocardial ischemia-reperfusion injury by activating AMPK-mediated M2 macrophage polarization.Mol Med.2025;31(1):98.Published 2025 Mar 13. [8] Chen S,Yao H,Lou Y,et al.Pharmacological upregulation of macrophage-derived itaconic acid by pubescenoside C attenuated myocardial ischemia-reperfusion injury.J Adv Res.2025;74:571-587. [9] Liu C,Sui H,Li Z,et al.THBS1 in macrophage-derived exosomes exacerbates cerebral ischemia-reperfusion injury by inducing ferroptosis in endothelial cells.J Neuroinflammation.2025;22(1):48.Published 2025 Feb 24. [10] Chik WI,Zhu L,Fan LL,et al.Saussurea involucrata:A review of the botany,phytochemistry and ethnopharmacology of a rare traditional herbal medicine.J Ethnopharmacol.2015;172:44-60. [11] Sebastian SA,Shah Y,Paul H,et al.Life's Essential 8 and the risk of cardiovascular disease:a systematic review and meta-analysis.Eur J Prev Cardiol.2025;32(5):358-373. [12] Zhao J,Cheng W,Dai Y,et al.Excessive accumulation of epicardial adipose tissue promotes microvascular obstruction formation after myocardial ischemia/reperfusion through modulating macrophages polarization.Cardiovasc Diabetol.2024;23(1):236.Published 2024 Jul 5. [13] Jiang Y,Yu W,Hu T,et al.Unveiling macrophage diversity in myocardial ischemia-reperfusion injury:identification of a distinct lipid-associated macrophage subset.Front Immunol.2024;15:1335333.Published 2024 Feb 21. [14] Lu H,Wang J,Chen Z,et al.Engineered Macrophage Membrane-Coated S100A9-siRNA for Ameliorating Myocardial Ischemia- Reperfusion Injury.Adv Sci(Weinh).2024;11(41):e2403542. [15] Zhuang L,Zong X,Yang Q,et al.Interleukin-34-NF-κB signaling aggravates myocardial ischemic/reperfusion injury by facilitating macrophage recruitment and polarization.EBioMedicine.2023;95:104744. [16] Cheng H,Jin A,Zhang Q,et al.KLF9-Mediated Transcriptional Promotion of HMGB2 Accelerates Cardiomyocyte Apoptosis, Inflammation,and Ferroptosis in Myocardial Ischemia/Reperfusion Injury.Cardiovasc Toxicol.2025;25(8):1181-1190. [17] Mutailifu D,Aini A,Aibibula A,et al.Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation.J Vis Exp.2025;(215):10.3791/67179.Published 2025 Jan 10. [18] Ma X,Fan Z,Peng J,et al.Ischemic Area-Targeting and Self-Monitoring Nanoprobes Ameliorate Myocardial Ischemia/Reperfusion Injury by Scavenging ROS and Counteracting Cardiac Inflammation.Adv Sci(Weinh).2025;12(11):e2414518. [19] Wang A,Li Y,Zhao F,et al.Cardioprotective Effect of Licorice Extract Against Myocardial Ischemia and Ischemia/Reperfusion Injury via Blocking Cardiac Inflammation by Sirt3/NLRP3.Phytother Res.2025;39(11):5198-5221.

点击下载PDF