符光戌,王 勇,郭钟义,谭登武,张 珍

目的：针对骨肉瘤术后局部复发与骨缺损问题，构建一种新型硒/钛金属有机框架（Se/Ti-MOF）复合水凝胶支架，以实现从“载体”到“活性治疗剂”的转变，避免传统载药系统对化疗药物的依赖。方法：采用水热法合成Se/Ti-MOF纳米颗粒，并通过物理共混－交联技术将其固载于明胶/壳聚糖基质中，制备Se/Ti-MOF@Gel/CS复合支架。利用SEM、XRD、FT-IR表征结构；测试力学、溶胀及降解性能；采用ICP-MS分析离子释放动力学；通过CCK-8、流式细胞术与Western Blot评估体外抗肿瘤活性及机制。结果：成功制备Se/Ti-MOF纳米颗粒（112.5±16.3 nm）及贯通多孔复合支架（孔径25.6±8.7μm）。Se/Ti-MOF使支架压缩模量由18.4±2.1 kPa提升至36.8±3.5 kPa。在pH 5.5条件下，支架溶胀与降解增强，Se/Ti离子协同释放加快。支架浸提液对143B和HOS细胞具有选择性毒性（IC50分别为4.197和5.624μg/mL），其机制涉及G2/M期阻滞、ROS爆发及线粒体凋亡通路激活。结论：Se/Ti-MOF@Gel/CS复合支架兼具良好力学性能、pH响应性及选择性抗肿瘤活性，通过调控细胞周期与ROS-线粒体凋亡通路发挥作用，为骨肉瘤术后“抗肿瘤－骨修复”一体化治疗提供了新平台。

硒/钛金属有机框架；水凝胶支架；骨肉瘤；细胞凋亡；活性氧

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